Title Antiseptikų veiksmingumo palyginimas veikiant Staphylococcus aureus infekuotą austą kraujagyslės protezą in vitro ir in vivo /
Translation of Title The comparison of the efficacy of the antiseptic solutions in treatment of Staphylococcus aureus infected woven vascular graft in vitro and in vivo.
Authors Stanevičiūtė, Elvyra
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Pages 104
Keywords [eng] Staphylococcus aureus ; in vitro ; in vivo ; vascular graft infection
Abstract [eng] Prosthetic vascular graft infection remains a great challenge in vascular surgery. Unsuccessful treatment of vascular prosthesis infection often results in limb amputations and death. A conservative treatment option – local wound irrigation with antiseptic solutions – it not yet a well established treatment method in clinical practice. Novel experimental in vitro and in vivo models of vascular graft infection were designed and disscussed in this dissertation. Three antiseptic solutions: 0.1% octenidine dihydrochloride, 10% povidone-iodine and 0.02% chlorhexidine digluconate, routinelly used in clinical practice, were used in the experiments. The experiments were performed with a woven polyester vascular graft, infected by biofilm-producing Staphylococcus aureus strains. In vitro experiments were designed to test antiseptics’ biofilm-disrupting activity and antimicrobial efficacy in a simulated wound environment. An innovative model of infected vascular graft was designed to test antiseptics’ ability to remove S. aureus biofilms from the surface of the vascular graft. In vivo experiments with Wistar rats were designed to evaluate antiseptics’ efficacy to treat prosthetic vascular graft infection by daily wound irrigations. The efficacy of different antiseptic solutions and the duration of the treatment needed to eradicate S. aureus from the wounds was evaluated and cytological and histological analysis of the perigraft tissues was performed.
Dissertation Institution Vilniaus universitetas.
Type Doctoral thesis
Language Lithuanian
Publication date 2019