| Title |
Structure of the DNA-bound spacer capture complex of a type II CRISPR-Cas system |
| Authors |
Wilkinson, Martin ; Drabavičius, Gediminas ; Šilanskas, Arūnas ; Gasiūnas, Giedrius ; Šikšnys, Virginijus ; Wigley, Dale B |
| DOI |
10.1016/j.molcel.2019.04.020 |
| Full Text |
|
| Is Part of |
Molecular cell.. Cambridge, MA : Cell Press. 2019, vol. 75, iss. 1, p. 90-101.. ISSN 1097-2765. eISSN 1097-4164 |
| Keywords [eng] |
CRISPR-Cas system ; spacer capture ; duplex DNA |
| Abstract [eng] |
The mechanism of spacer capture by type II CRISPR-Cas systems remains enigmatic. Wilkinson et al. report cryo-EM structures of three macromolecular assemblies of the Cas1, Cas2, and Csn2 proteins. All three complexes contain duplex DNA running along a central channel. A filamentous form contains a continuous duplex and smaller units contain shorter sections of ∼30 bp. |
| Published |
Cambridge, MA : Cell Press |
| Type |
Journal article |
| Language |
English |
| Publication date |
2019 |
