| Title |
Skin extracellular matrix components accelerate the regenerative potential of Lin-cells |
| Authors |
Ramanauskaitė, Giedrė ; Žalalytė, Dovilė ; Kašėta, Vytautas ; Vaitkuvienė, Aida ; Kalėdienė, Lilija ; Biziulevičienė, Genė |
| DOI |
10.2478/s11535-013-0283-9 |
| Full Text |
|
| Is Part of |
Central European journal of biology.. Warsaw : Versita. 2014, Vol. 9, no. 4, p. 367-373.. ISSN 1895-104X |
| Keywords [eng] |
Lineage negative cells ; BALB/c mouse ; Proliferation ; Migration ; In vitro |
| Abstract [eng] |
Due to their unique properties, bone marrow-derived Lin− cells can be used to regenerate damaged tissues, including skin. The objective of our study was to determine the influence of the skin tissue-specific microenvironment on mouse Lin− cell proliferation and migration in vitro. Cells were analyzed for the expression of stem/progenitor surface markers by flow cytometry. Proliferation of MACS-purified cells in 3D cultures was investigated by WST-8 assay. Lin− cell migration was evaluated by in vitro scratch assay. The results obtained show that basement membrane matrix is more effective for Lin− cell proliferation in vitro. However, type I collagen matrix better enhances the re-epithelization process, that depends on the cell migratory properties. These studies are important for preparing cells to be used in transplantation. |
| Published |
Warsaw : Versita |
| Type |
Journal article |
| Language |
English |
| Publication date |
2014 |
