| Title |
Formation and stoichiometry of CRISPR-Cascade complexes with varying spacer lengths revealed by native mass spectrometry / |
| Authors |
Wittig, Sabine ; Songailienė, Inga ; Schmidt, Carla |
| DOI |
10.1021/jasms.9b00011 |
| Full Text |
|
| Is Part of |
Journal of the American Society for Mass Spectrometry.. New York : Springer. 2020, vol. 31, iss. 3, p. 538-546.. ISSN 1044-0305. eISSN 1879-1123 |
| Keywords [eng] |
CRISPR ; complex heterogeneity ; crRNA ; native mass spectrometry ; stoichiometry ; type I-E Cascade complex |
| Abstract [eng] |
The adaptive immune system of bacteria and archaea against viral DNA is based on clustered, regularly interspaced, short palindromic repeats (CRISPRs) which are encoded in the host genome and translated into CRISPR RNAs (crRNAs) containing single spacer sequences complementary to foreign DNA. crRNAs assemble with CRISPR-associated (Cas) proteins forming surveillance complexes that base-pair with viral DNA and mediate its degradation. As specificity of degradation is provided by the crRNA spacer sequence, genetic engineering of the CRISPR system has emerged as a popular molecular tool, for instance, in gene silencing and programmed DNA degradation. Elongating or shortening the crRNA spacer sequence are therefore promising ventures to modify specificity toward the target DNA. However, even though the stoichiometry of wild-type complexes is well established, it is unknown how variations in crRNA spacer length affect their stoichiometry. The CRISPR-associated antiviral defense surveillance complexes of Streptococcus thermophilus (StCascade complexes) contain crRNA and five protein subunits. Using native mass spectrometry, we studied the formation and stoichiometry of StCascade complexes assembled on a set of crRNAs with different spacer lengths. We assigned all relevant complexes and gained insights into the stoichiometry of the complexes as well as their preferred assembly. We found that stable complexes, which incorporate or lose a (Cas7)2(Cse2)1-module, assemble on crRNA varied in length by 12-nucleotide units, while varying crRNA length in six-nucleotide units results in heterogeneous mixtures of complexes. Combining our results from the various variants, we generated an assembly pathway revealing general features of I-E type Cascade complex formation. |
| Published |
New York : Springer |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
