| Title |
Biocompatibility investigation of hybrid organometallic polymers for sub-micron 3D printing via laser two-photon polymerisation / |
| Authors |
Balčiūnas, Evaldas ; Dreižė, Nadežda ; Grubliauskaitė, Monika ; Urnikytė, Silvija ; Šimoliūnas, Egidijus ; Bukelskienė, Virginija ; Valius, Mindaugas ; Baldock, Sarah J ; Hardy, John G ; Baltriukienė, Daiva |
| DOI |
10.3390/ma12233932 |
| Full Text |
|
| Is Part of |
Materials.. Basel : MDPI. 2019, vol. 12, iss. 23, art. no. 3932, p. [1-12].. ISSN 1996-1944. eISSN 1996-1944 |
| Keywords [eng] |
bioactive surfaces ; biomaterials ; hybrid organometallic polymers ; laser two-photon polymerisation ; tissue engineering |
| Abstract [eng] |
Hybrid organometallic polymers are a class of functional materials which can be used to produce structures with sub-micron features via laser two-photon polymerisation. Previous studies demonstrated the relative biocompatibility of Al and Zr containing hybrid organometallic polymers in vitro. However, a deeper understanding of their effects on intracellular processes is needed if a tissue engineering strategy based on these materials is to be envisioned. Herein, primary rat myogenic cells were cultured on spin-coated Al and Zr containing polymer surfaces to investigate how each material affects the viability, adhesion strength, adhesion-associated protein expression, rate of cellular metabolism and collagen secretion. We found that the investigated surfaces supported cellular growth to full confluency. A subsequent MTT assay showed that glass and Zr surfaces led to higher rates of metabolism than did the Al surfaces. A viability assay revealed that all surfaces supported comparable levels of cell viability. Cellular adhesion strength assessment showed an insignificantly stronger relative adhesion after 4 h of culture than after 24 h. The largest amount of collagen was secreted by cells grown on the Al-containing surface. In conclusion, the materials were found to be biocompatible in vitro and have potential for bioengineering applications. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2019 |
