Title |
Dual- and single-shot susceptibility ratio measurements with circular polarizations in second-harmonic generation microscopy / |
Authors |
Golaraei, Ahmad ; Kontenis, Lukas ; Karunendiran, Abiramy ; Stewart, Bryan A ; Barzda, Virginijus |
DOI |
10.1002/jbio.201960167 |
Full Text |
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Is Part of |
Journal of biophotonics.. Weinheim : Wiley - VCH Verlag GmbH & Co. KGaA. 2020, vol. 13, no. 4, art. no. e201960167, p. [1-9].. ISSN 1864-063X. eISSN 1864-0648 |
Keywords [eng] |
muscle ; nonlinear optical polarimetry ; second-harmonic generation microscopy ; second-order susceptibility |
Abstract [eng] |
Polarization-resolved second harmonic generation (P-SHG) microscopy is a technique capable of characterizing nonlinear optical properties of noncentrosymmetric biomaterials by extracting the nonlinear susceptibility tensor components ratio χð2Þ0 zzz =χ ð2Þ0 zxx , with z-axis parallel and x-axis perpendicular to the C6 symmetry axis of molecular fiber, such as a myofibril or a collagen fiber. In this paper, we present two P-SHG techniques based on incoming and outgoing circular polarization states for a fast extraction of χ ð2Þ0 zzz =χ ð2Þ0 zxx : A dual-shot configuration where the SHG circular anisotropy generated using incident right- and left-handed circularly-polarized light is measured; and a single-shot configuration for which the SHG circular anisotropy is measured using only one incident circular polarization state. These techniques are used to extract the χ ð2Þ0 zzz =χ ð2Þ0 zxx of myosin fibrils in the body wall muscles of Drosophila melanogaster larva. The results are in good agreement with values obtained from the double Stokes-Mueller polarimetry. The dual- and single-shot circular anisotropy measurements can be used for fast imaging that is independent of the in-plane orientation of the sample. They can be used for imaging of contracting muscles, or for high throughput imaging of large sample areas. |
Published |
Weinheim : Wiley - VCH Verlag GmbH & Co. KGaA |
Type |
Journal article |
Language |
English |
Publication date |
2020 |
CC license |
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