| Title |
Efficiency of granulocyte colony-stimulating factor immobilized on magnetic microparticles on proliferation of NFS-60 cells / |
| Authors |
Popov, Anton ; Stirkė, Arūnas ; Bakutė, Neringa ; Brasiūnas, Benediktas ; Ramanavičius, Arūnas ; Ramanavičienė, Almira |
| DOI |
10.1016/j.colsurfa.2019.123580 |
| Full Text |
|
| Is Part of |
Colloids and surfaces : A-physicochemical and engineering aspects.. Amsterdam : Elsevier B.V.. 2019, vol. 578, art. no. 123580, p. [1-7].. ISSN 0927-7757. eISSN 1873-4359 |
| Keywords [eng] |
Granulocyte colony-stimulating factor ; Magnetic particles ; Biomodification ; Cell proliferation |
| Abstract [eng] |
Magnetic particles are promising carriers for targeted drug delivery. Granulocyte colony-stimulating factor (G-CSF) is a cytokine which stimulates neutrophil proliferation process. In this study, G-CSF molecules were successfully covalently immobilized on magnetic silica gel beads (MagBs) surface. Comparison of free and on MagBs immobilised G-CSF (MagBs-G) on proliferation efficiency and viability of mouse myeloid cell line NFS-60 was performed using XTT assay. Cytotoxic or stimulation effects of used MagBs on NFS-60 cell proliferation were not observed. However, the positive effect on proliferation of cells was noticed increasing the concentration of MagB-G in the test wells from 0.5 μg/ml to 50 μg/ml. Proliferation of cells in the presence of 50 μg/ml MagBs-G (2.7 μg/ml G-CSF) resulted in maximal proliferation response, which was reached using free G-CSF. Comparing the concentration of G-CSF that gives half-maximal response of free (3.6 pM) and on MagBs immobilized (11.4 nM) G-CSF can be concluded that immobilized G-CSF possessed the same efficiency as free G-CSF molecules, however the higher amount of immobilized G-CSF is required. |
| Published |
Amsterdam : Elsevier B.V |
| Type |
Journal article |
| Language |
English |
| Publication date |
2019 |
