| Title |
A rapid method for the selection of amidohydrolases from metagenomic libraries by applying synthetic nucleosides and a uridine auxotrophic host / |
| Authors |
Urbelienė, Nina ; Meškienė, Rita ; Tiškus, Matas ; Stanislauskienė, Rūta ; Aučynaitė, Agota ; Laurynėnas, Audrius ; Meškys, Rolandas |
| DOI |
10.3390/catal10040445 |
| Full Text |
|
| Is Part of |
Catalysts.. Basel : MDPI AG. 2020, vol. 10, iss. 4, art. no. 445, p. [1-12].. eISSN 2073-4344 |
| Keywords [eng] |
Amidase ; Amidohydrolase ; High-throughput screening ; Metagenomic libraries ; Selection method |
| Abstract [eng] |
In this study, the development of a rapid, high-throughput method for the selection of amide-hydrolysing enzymes from the metagenome is described. This method is based on uridine auxotrophic Escherichia coli strain DH10B ∆pyrFEC and the use of N4-benzoyl-2’-deoxycytidine as a sole source of uridine in the minimal microbial M9 medium. The approach described here permits the selection of unique biocatalysts, e.g., a novel amidohydrolase from the activating signal cointegrator homology (ASCH) family and a polyethylene terephthalate hydrolase (PETase)-related enzyme. |
| Published |
Basel : MDPI AG |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
