| Title |
Biohibridinių metalas-baltymas kompleksų kūrimas ir tyrimai / |
| Translation of Title |
Synthesis and study of biohybrid metal-protein complexes. |
| Authors |
Mečinskas, Tautvilas |
| Full Text |
|
| Pages |
39 |
| Abstract [eng] |
Biochemical enzyme cascade is a series of chemical reactions in which the products of one reaction are consumed in the next reaction. If one could organize all the necessary enzymes for the reaction in close quarters this could possibly lead to more effective cascade reactions. One way of organizing enzymes is by fusing them on barcoded nanowire matrices. This could be achieved by tayloring enzyme molecules with genetically engineered proteins for inorganics (GEPIs). My assignment was to characterise possible nanowire candidates for these biohybrid complexes using AFM and examine silver binding characteristics of GEPI taylored streptavidin using SERS. I could not realiably characterise nanowires because the interaction between AFM probe and nanowires was to interfering. Also the nanowires used to aggregate and it was difficult to separate them using ultrasound. 15nm diameter nanowires aggregated more thant 30nm diameter nanowires. Streptavidin taylored with Ag binding GEPIs showed stronger interaction with Ag electrode surface than ordinary streptavidin. Also this modified streptavidin was capable of binding with biotin. This proves that added oligopeptide chains did not negatively affect the chemical structure of streptavidin. |
| Type |
Master thesis |
| Language |
Lithuanian |
| Publication date |
2014 |
