Title Isolation and characterization of vB_ArS-ArV2 - first Arthrobacter sp. infecting bacteriophage with completely sequenced genome /
Authors Šimoliūnas, Eugenijus ; Kalinienė, Laura ; Stasilo, Miroslav ; Truncaitė, Lidija ; Zajančkauskaitė, Aurelija ; Staniulis, Juozas Benediktas ; Nainys, Juozas ; Kaupinis, Algirdas ; Valius, Mindaugas ; Meškys, Rolandas
DOI 10.1371/journal.pone.0111230
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Is Part of PLoS ONE [Elektroninis išteklius].. San Francisko : Public Library of Science. 2014, Vol. 9, iss. 10, art. no e111230 [1-11].. ISSN 1932-6203
Keywords [eng] Phage ; Soil ; Rhodococcus ; Proteins ; DNA ; Globiformis ; Evolution ; Tail ; Recombination
Abstract [eng] This is the first report on a complete genome sequence and biological characterization of the phage that infects Arthrobacter. A novel virus vB_ArS-ArV2 (ArV2) was isolated from soil using Arthrobacter sp. 68b strain for phage propagation. Based on transmission electron microscopy, ArV2 belongs to the family Siphoviridae and has an isometric head (~63 nm in diameter) with a non-contractile flexible tail (~194×10 nm) and six short tail fibers. ArV2 possesses a linear, double-stranded DNA genome (37,372 bp) with a G+C content of 62.73%. The genome contains 68 ORFs yet encodes no tRNA genes. A total of 28 ArV2 ORFs have no known functions and lack any reliable database matches. Proteomic analysis led to the experimental identification of 14 virion proteins, including 9 that were predicted by bioinformatics approaches. Comparative phylogenetic analysis, based on the amino acid sequence alignment of conserved proteins, set ArV2 apart from other siphoviruses. The data presented here will help to advance our understanding of Arthrobacter phage population and will extend our knowledge about the interaction between this particular host and its phages.
Published San Francisko : Public Library of Science
Type Journal article
Language English
Publication date 2014