| Title |
Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae / |
| Authors |
Slibinskas, Rimantas ; Ražanskas, Raimundas ; Zinkevičiūtė, Rūta ; Čiplys, Evaldas |
| DOI |
10.1186/1477-5956-11-36 |
| Full Text |
|
| Is Part of |
Proteome science.. London : BioMed Central Ltd. 2013, vol. 11, Art. No. 36.. ISSN 1477-5956 |
| Abstract [eng] |
The drawback of NEPHGE-based 2DE method is its failure to detect some highly acidic proteins. The advantage of NEPHGE is higher protein capacity with good reproducibility and quality of spots at high protein load. Conclusions Comparison of broad range (pH 3–10) gradient-based 2DE methods suggests that NEPHGE-based method is preferable over IPG (Invitrogen) 2DE method for the analysis of basic proteins. Nevertheless, the narrow range (pH 4–7) IPG technique is a method of choice for the analysis of acidic proteins. |
| Published |
London : BioMed Central Ltd |
| Type |
Journal article |
| Language |
English |
| Publication date |
2013 |
