| Title |
Synthesis of hepatitis B virus surface protein derivates in yeast S. cerevisiae / |
| Translation of Title |
Hepatito B viruso paviršiaus baltymų darinių sintezė mielėse s. cerevisiae. |
| Authors |
Bulavaitė, Aistė ; Sabaliauskaitė, Rasa ; Staniulis, Juozas Benediktas ; Sasnauskas, Kęstutis |
| Full Text |
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| Is Part of |
Biologija. 2006, nr. 4, p. 49-53.. ISSN 1392-0146 |
| Keywords [eng] |
Hepatitis B virus ; HBV surface proteins ; PreS1 ; Virus-like particles |
| Abstract [eng] |
HBV surface proteins PreS1[13–59]-S, PreS1[20–59]-S, PreS1[30–59]-S, PreS1[40–59]-S, PreS1[50–59]-S, PreS1[90–119]-S were produced in S.cerevisiae and purified. Electron microscopy suggested spherical virus-like particle formation for all the proteins except PreS1[90–119]-S. The PreS1[90–119] sequence was demonstrated to decrease protein solubility. Proteins are suitable for Tupaia primary hepatocyte binding investigations, diagnostic products and vaccine candidate development. |
| Type |
Journal article |
| Language |
English |
| Publication date |
2006 |
