Title Quinolone resistance among Salmonella enterica and Escherichia coli in Lithuania /
Translation of Title Lietuvoje išskirtų Escherichia coli ir Salmonella enterica padermių atsparumas chinolonams.
Authors Šeputienė, Vaida ; Povilonis, Justas ; Ružauskas, Modestas ; Virgailis, Marius ; Žlabys, Pranas ; Sužiedėlienė, Edita
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Is Part of Biologija. 2006, Nr. 3, p. 74-78.. ISSN 1392-0146
Keywords [eng] Escherichia coli ; Salmonella enterica ; Quinolone resistance ; GyrA ; ParC
Abstract [eng] Lithuanian isolates of Escherichia coli and Salmonella enterica from animals and humans were examined for resistance to quinolones, fluoroquinolones and for resistance-associated mutations. 9% of S. enterica from animals and 4% of isolates from clinical samples of humans were resistant to nalidixic acid and susceptible to fluoroquinolones. DNA analysis of nalidixic acid-resistant S. enterica strains from animals revealed a single mutation at codon 83 (Ser→Phe) in gyrA gene, whereas resistant clinical strains contained a single gyrA mutation at codon 87 (Asp→Tyr). 10% of human isolates of E. coli were resistant to nalidixic acid and ciprofloxacin. 22% of E. coli isolates from calves were resistant to nalidixic acid. 40% and 20% of E. coli isolates from pigs were resistant to nalidixic acid and to fluoroquinolones, respectively. E. coli isolates of animal and human origin analyzed for nalidixic acid resistance-associated mutations carried single mutations at codon 83 (Ser→Leu) or at codon 87 (Asp→Tyr) in gyrA gene. Fluoroquinolone-resistant E. coli isolates from calves and humans carried multiple mutations within gyrA (83Ser→Leu, 87Asp→Gly or Asn) and parC (80Ser→Ile or Arg, 84Glu→Val or Lys) genes.
Type Journal article
Language English
Publication date 2006