| Title |
Paprastosios eglės (Picea Abies (L.) Karst.) pliusinių medžių klonų genetinės įvairovės ir tapatumo įvertinimas APPD metodu / |
| Translation of Title |
Estimation of genetic diversity and indentity in plus tree clones of Norway spruce (Picea abies (L.) Karst) using RAPD method. |
| Authors |
Staniulytė, Ramunė ; Žvingila, Donatas ; Kuusienė, Sigutė |
| Full Text |
|
| Is Part of |
Miškininkystė. 2004, Nr. 1, p. 5-12.. ISSN 1392-2041 |
| Keywords [eng] |
RAPD ; Plus tree clone ; Picea abies |
| Abstract [eng] |
The RAPD (random amplified polymorphic DNA) was used to assess genetic diversity and to identify genotype specific markers in Norway spruce (Picea abies (L.) Karst.) plus tree clones. Twenty-three genotypes originally collected from different localities of Lithuania and now growing in a clonal archive at Dubrava experimental-training forest enterprise were analyzed by using 20 oligonucleotide primers. A total of 299 RAPD bands were scored, of which 243 were polymorphic. The estimated genetic distance varied from 0.2905 to 0.5000. These results confirm that studied P. abies clones exhibit a high genetic variation. Fourteen genotype-specific DNA bands were identified. The RAPD technique was also used to check the clonal identity. We compared RAPD profiles of 5 ramets for each of 3 plus tree. Some differences in the RAPD patterns resulting from mis-planting or mis-labeling of cuttings were found. We also established a reference library of RAPD fingerprints for 23 clones, which could serve as a reference source in cases of litigation concerning clone identity. This study confirmed that the RAPD technique is a useful tool for Picea abies genetic analysis and for forestry service in managing propagation operations. Also it could be useful for Norway spruce genetic resources analysis and conservation in Lithuania. |
| Type |
Journal article |
| Language |
Lithuanian |
| Publication date |
2004 |
