| Title |
A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample / |
| Authors |
Eidukevičius, Rimantas ; Characiejus, Dainius ; Janavicius, Ramūnas ; Kazlauskaitė, Nijolė ; Pašukonienė, Vita ; Mauricas, Mykolas ; Den Otter, Willem |
| DOI |
10.1186/1471-2407-5-122 |
| Full Text |
|
| Is Part of |
BMC cancer [Elektroninis išteklius]. 2005, vol. 5, art. no 122, p. [1-11].. ISSN 1471-2407 |
| Keywords [eng] |
Potential doubling time ; Kinetic measurements ; Neck-cancer ; T-pot ; Radiotherapy ; Ki-67 ; Parameters ; Protein ; Future ; Proliferation |
| Abstract [eng] |
6 +/- 0.9 h, respectively). GF in tumours at day 10 was lower than GF at day 4 (54.2 +/- 7.7% vs. 79.2 +/- 5.9%, p = 0.0003). Approximate values of T-C and GF of cultured Jurkat cells were 23.9 h and 79.3%, respectively. Conclusion: The proposed method is relatively simple and permits estimation of the cell cycle parameters, including T-C and GF, from a single tumour sample after labelling with BrdUrd. We have shown that this method may be useful in preclinical studies, allowing estimation of changes in GF during growth of murine tumours. Experiments with human Jurkat cells suggest that the proposed method might also prove suitable for measurement of cell kinetics in human tumours. Development of suitable software enabling more objective interpretation of the DNA profile in this method would be desirable. |
| Type |
Journal article |
| Language |
English |
| Publication date |
2005 |
| CC license |
|
