| Title |
Chloramphenicol determination in milk by liquid chromatography - tandem mass spectrometry / |
| Translation of Title |
Chloramfenikolio nustatymas piene skysčių chromatografijos-dvigubos masių spektrometrijos metodu. |
| Authors |
Tamošiūnas, Vytautas ; Petraitis, Julijonas ; Padarauskas, Audrius |
| Full Text |
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| Is Part of |
Chemija.. Vilnius : Lietuvos mokslų akademijos l-kla. 2006, T. 17, Nr. 2-3, p. 25-29.. ISSN 0235-7216 |
| Keywords [eng] |
high-performance liquid chromatography ; tandem mass spectrometry ; chloramphenicol ; milk products |
| Abstract [eng] |
A high-performance liquid chromatography - tandem mass spectrometry (HPLC— MS—MS) method was developed for the determination of chloramphenicol (CAP) in milk products. Mass spectral acquisition was done using electrospray ionization in the negative ion mode applying multiple reaction monitoring of two diagnostic transition reactions for CAP (m/z 321->152 and m/z 321->257). Milk samples were extracted with ethyl acetate and evaporated to dryness, follovved by a clean-up step using the liquįd—liquid extraction with carbon tetrachloride / hexane (1:1 v/v) mixture. The calibration curve showed a good linearity in the concentration range from 0.02 to 1.0 ųg/kg with the correlation coefficient above 0.995. The method gave a decision limit and a detection capability of 0.050 and 0.066 ųg/kg, respectively. The mean recoveries of CAP from the milk samples spiked at 0.1-0.45 ųg/kg were in the range of 86-92%. The applicability of this technique was demonstrated by analysis of milk products. |
| Published |
Vilnius : Lietuvos mokslų akademijos l-kla |
| Type |
Journal article |
| Language |
English |
| Publication date |
2006 |
