| Abstract [eng] |
Cells in the organism are surrounded by an environment that is both chemically and mechanically complex and distinct. There, they constantly interact with the extracellular matrix (substrate), which sends signals to the nucleus in a multitude of ways, activating the expression of genes responsible for cell migration, proliferation, differentiation or apoptosis. Using the knowledge about such biological contacts, various artificial surfaces are manufactured which solely by their physical, chemical and mechanical properties stimulate the desired response and thus enable to control the fate of cells. One of the practical areas where such cell-substrate interaction studies can be used is the construction of artificial tissues. In this work we analysed various FFF 3D-printed polylactic acid (PLA) scaffold modifications’ impact on dental pulp stem cells‘ (DPSC) fate for creation of functional artificial bone tissue. Rat DPSCs were selected as a model. PLA scaffolds were modified by altering their topography, chemical composition, and surface coating with proteins in order to determine the impact of each substrate modification on cell adhesion, proliferation and differentiation. The main study focus was the control of DPSC osteogenesis process; to this end, molecular cell differentiation studies and analysis of cell-formed, bone tissue-specific extracellular environment were performed. Moreover, the manufactured constructs (DPSC-substrate) were evaluated as possible artificial bone tissue – their impact on the recipient in case of implantation was determined.This scientific work significantly contributes to bone regenerative medicine progress. |
