| Title |
Investigation and modeling of complex biocatalytic and bioelectrocatalytic processes / |
| Translation of Title |
Sudėtingų biokatalizinių bei bioelektrokatalizinių procesų tyrimas ir modeliavimas. |
| Authors |
Laurynėnas, Audrius |
| Full Text |
|
| Pages |
183 |
| Keywords [eng] |
biocatalysis ; bioelectrocatalysis ; biochemical kinetics ; oxidoreductases ; theoretical models |
| Abstract [eng] |
The goal of the thesis is to develop a method for the calculation of reaction rate parameters of complex homogeneous biochemical reactions and to apply it for the investigation of oxidation/reduction processes catalyzed by enzymes in homogeneous media and on surfaces. In the first part of the thesis a method is developed for a calculation of reaction rate parameters from experimental data. In the second part this method is applied for the investigation of catalysis mechanism of laccase from Didymocrea sp. J6 in homogeneous media and on the surface. It was found that this laccase is capable for unusual direct electron transfer from electrode to the T2/T3 active center and mechanism of inhibition with fluoride ions was deduced. In the third part of the thesis, a reduction mechanism of the compound II of the peroxidase from Coprinus cinereus was investigated. It was found that in the first step of the catalysis electron is transferred and afterwards proton transfer follows. The reaction rate constants were explained in terms of Marcus electron transfer theory and nonadiabatic proton transfer theory. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Doctoral thesis |
| Language |
English |
| Publication date |
2020 |
