| Abstract [eng] |
In Plantae information about environmental conditions can be transmitted through electrical signalling. Action potentials of plants possess the main characteristics of mammalian action potentials: they are all-or-none responses with inherent excitation threshold, duration and shape. Despite the inherent characteristics, the parameters of plant action potentials are altered in response to environmental cues and chemical compounds. It is known that environmental amino acids can modulate or elicit action potential generation in plants by binding to glutamate receptor-like channels. The effect of amino acids on the electrical signalling parameters of a separate intact plant cell are yet to be elucidated. The dissertation describes the effect pattern of amino acids and a synthetic ionotropic glutamate receptor channel agonist NMDA on the action potential parameters of a single Charophyta cell via microelectrode technique in current- and voltage-clamp modes, also suggests standardised evaluation methods for action potentials in plants. Reported dose-dependent modulations, described inhibition of the effect by ionotropic glutamate receptors antagonists supports the functioning of glutamate receptor-like channels in Characeaen, confirms the modulation of action potentials as a possible response to environmental amino acids and stresses the importance of multi-parametrical analysis of plant electrical signals. |
