| Abstract [eng] |
The Synthesis of Plant Allergens in Nicotiana benthamiana Transient Expression System Allergy is a strong immune system reaction to certain substances that normally do not cause any effect to a human body. Any substance that contains protein can cause allergy. Currently, the most popular method of diagnosing allergies is based on the use of extracts from natural materials. It is known that one source of allergies is a mixture of different components and each component has different characteristics. Often cross-interactions of IgE antibodies with homologous proteins occur in diagnostics. The extracts used may also contain components from other sources of allergens. These aspects complicate the standardization of extracts and the diagnosis of allergic diseases. The use of individual proteins could increase the sensitivity of allergy-detecting tests, but it requires high levels of allergens. This problem can be solved using genetic engineering techniques. Plant expression systems are becoming an increasingly popular platform for the synthesis of various therapeutic proteins due to high yields, affordability and suitable post-transduction modifications. We used transient expression system of black alder (lot. Alnus glutinosa) Aln g 1, olive (lot. Olea europaea) Ole e 1, kentucky bluegrass (lot. Poa pratensis) Poa p 1, pineapple (lot. Ananas comosus) Ana c 2, peach (lot. Prunus persica) Pru p 1, hazel (lot. Corylus avellana) Cor a 2, sunflower (lot. Helianthus annuus) Hel a 3, mugword (lot. Artemisia vulgaris) Art v 6 proteins production that is based on tobacco mosaic virus in Nicotiana benthamiana plants. |
