| Abstract [eng] |
Post-translational modification of histones is one of the epigenetic mechanisms leading to DNA sequence-independent phenotypic change. The "histone code" is recognized and interpreted by protein complexes that include enzymes which either add, recognize or remove epigenetic marks. Chromatin immunoprecipitation (ChIP) method is used to study protein – DNA interactions in vivo. ChIP uses antibodies that recognize the histone modifications to extract DNA, which is later analyzed using PCR or DNA sequencing methods. In this study, ChIP was used to investigate the effect of demethylase induced histone modifications on gene expression in cancer cells. Changes in gene expression impacted by a demethylase inhibitor were evaluated using qPCR and RT-qPCR methods, and the results were compared to the data found in literature. During the study, it was found that the demethylase inhibitor and the interfering RNA mentioned in literature affects the histone demethylation differently and in turn affects the expression of the studied genes differently. |
