| Abstract [eng] |
Associations Between SIRT1 rs3758391 and rs3818292 Gene Polymorphisms and Age-Related Macular Degeneration Age-related macular degeneration (AMD) is a progressive neurodegenerative eye disease which damages the macula in the retina. The macula is responsible for central vision. AMD is one of the main causes of vision loss especially in developed countries. Many genetic variants have been consistently assessing to contribute with AMD over the last decade. The goal of this study is to determine the correlation between SIRT1 rs3758391 and rs3818292 gene polymorphisms and the occurrence of age-related macular degeneration. 756 patients with AMD and 323 healthy control group subjects were examined. DNA was extracted from peripheral blood leukocytes using DNA salting-out method. Genotyping was carried out using real-time polymerase chain reaction (RT-PCR) method. Statistical analysis was performed with „SPSS version 20.0“. Conclusions. AG and GG genotypes and each alelle A at SIRT rs3818292 are associated with increased odds of exudative AMD. Moreover, AG genotype is associated with increased risk of early AMD. AG and GG genotypes and each alelle G at SIRT rs3818292 are associated with increased risk of early and exudative AMD in males and in older age. However, in females no statistically results were dound. SIRT1 rs3758391 each alelle C is associated with increased risk of exudative AMD. Additionally, CC genotype and each alelle C is associated with increased risk of exudative AMD in younger age. |
