| Abstract [eng] |
Analysis of Inflammatory miRNAs for Diagnosis of Chronic Periodontitis Chronic periodontitis (CP) is a highly prevalent inflammatory disease affecting 20-50% of the human population worldwide. When left untreated, CP increases the risk of some other systemic diseases such as diabetes, rheumatoid arthritis (RA) and cancer. Due to the asymptomatic onset of the disease and out-dated diagnostic methods, most of the cases are diagnosed at an advanced stage when the supporting tissues of the tooth are already irreversibly damaged. The development of CP is highly associated with a number of differentially expressed miRNAs that may be identified in the damaged oral epithelium and periodontal tissues. Novel methods based on the assessment of circulating miRNA level in human bodily fluids may be applied as a non-invasive tool for early CP diagnosis. The aim of the current study was to evaluate miRNA expression profile in periodontal tissues by means of miRNA microarray technology and reverse transcription PCR technique to select CP-associated miRNAs and analyse them in bodily fluids (saliva and blood plasma) and to evaluate their diagnostic potential. The expression of miR-145-5p (P <0.001) and -140-3p (P = 0.006) was significantly upregulated in CP-affected tissues. Moreover, the expression of miR-145-5p (P = 0.016) was associated with advanced stage CP, while upregulation of miR-195-5p (P = 0.029) was specific for moderate stage of CP, compared with healthy controls. Decreased level of miR-140-3p (P = 0.049) was observed in plasma samples from CP patients. The highest discriminatory power was assessed for combination of all four salivary miRNAs (AUC = 0.66; P = 0.027) and plasma miR-145-5p /-195-5p (AUC = 0.64; P = 0.040). The current study has shown that four miRNAs play an important role in the pathogenesis of CP. Selected miRNAs were found to be associated with clinical-pathological factors of CP and other modulating factors of the disease. However, further investigations are necessary to confirm these findings. |
