| Abstract [eng] |
More than 425 million people worldwide suffer from diabetes. Elevated blood glucose (hyperglycemia) is the most prominent symptom of the disease (DeFronzo et al., 2015). Poor glycemic control in diabetic patients increases the risk of various micro- and macrovascular complications of diabetes, such as retinopathy, neuropathy, nephropathy (Lima et al., 2019). There are several methods used to evaluate DNA damage associated with diabetes and its complications. One of them is the sister chromatid exchange (SCE) assay. SCE frequency increases when cells are exposed to various genotoxic carcinogens (Salimi et al., 2016). 4-Nitroquinoline 1-oxide (4NQO), a potent carcinogen that induces oxidative DNA damage, was used in this work (Ikenaga et al., 1975). Statistical analysis showed a dependence of the amount of uninduced SCE on demographic and clinical patient parameters. It has been observed that lower SCE levels are common in patients with type II diabetes and higher SCE levels are common in elderly patients. In a 4NQO genotoxicity study, the dependence of SCE levels on demographic and clinical patient parameters showed higher levels of induced SCE in lymphocytes from patients with type II diabetes. In cultures not affected by 4NQO, variation in RI values showed dependence on diagnosis (cells in patients with type 2 diabetes divided more slowly) and age (cells in older patients divided more slowly). |
