| Title |
Macro-, micro- and nano-roughness of carbon-based interface with the living cells:towards a versatile bio-sensing platform / |
| Authors |
Golubewa, Lena ; Rehman, Hamza ; Kulahava, Tatsiana ; Karpič, Renata ; Baah, Marian ; Kaplas, Tommy ; Shah, Ali ; Malykhin, Sergei ; Obraztsov, Alexander ; Rutkauskas, Danielis ; Jankunec, Marija ; Matulaitienė, Ieva ; Selskis, Algirdas ; Denisov, Andrei ; Svirko, Yuri ; Kuzhir, Polina |
| DOI |
10.3390/s20185028 |
| Full Text |
|
| Is Part of |
Sensors.. Basel : MDPI AG. 2020, vol. 20, iss. 18, art. no. 5028, p. 1-14.. ISSN 1424-8220 |
| Keywords [eng] |
pyrolytic carbon ; graphene ; graphene nanowalls ; black silicon ; glioma cells ; biocompatibility |
| Abstract [eng] |
Integration of living cells with nonbiological surfaces (substrates) of sensors, scaffolds,and implants implies severe restrictions on the interface quality and properties, which broadlycover all elements of the interaction between the living and artificial systems (materials, surface modifications, drug-eluting coatings, etc.). Substrate materials must support cellular viability,preserve sterility, and at the same time allow real-time analysis and control of cellular activity.We have compared new substrates based on graphene and pyrolytic carbon (PyC) for the cultivation of living cells. These are PyC films of nanometer thickness deposited on SiO2and black silicon and graphene nanowall films composed of graphene flakes oriented perpendicular to the Si substrate.The structure, morphology, and interface properties of these substrates are analyzed in terms of their biocompatibility. The PyC demonstrates interface biocompatibility, promising for controllingcell proliferation and directional intercellular contact formation while as-grown graphene walls possess high hydrophobicity and poor biocompatibility. By performing experiments with C6glioma cells we discovered that PyC is a cell-friendly coating that can be used without poly-l-lysineor other biopolymers for controlling cell adhesion. Thus, the opportunity to easily control the physical/chemical properties and nanotopography makes the PyC films a perfect candidate for the development of biosensors and 3D bioscaffolds. |
| Published |
Basel : MDPI AG |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
