| Title |
Scanning Electrochemical Microscopy for Human Myocardium-Derived Mesenchymal Stem Cells Investigation / |
| Translation of Title |
Skenuojančios elektrocheminės mikroskopijos taikymas žmogaus širdies mezenchiminių kamieninių ląstelių tyrimuose. |
| Authors |
Petronienė, Jūratė Jolanta |
| Full Text |
|
| Pages |
135 |
| Keywords [eng] |
Scanning Electrochemical Microscopy ; Saccharomyces cerevisiae cells ; human heart left ventricle myocardium-derived mesenchymal stem cells ; ,menadione ; Fast Fourier Transform (FFT) electrochemical impedance |
| Abstract [eng] |
Scanning Electrochemical Microscopy (SECM) have a wide application, expecially in a field of science of the surface electrochemical activity. It takes a few minutes to record a Electrochemical Impedance spectra (EIS) by conventional devices, but Fast Fourier Transform Impedansometer (FFTS) can register EIS in a seconds, because this devices is not collect frequency by frequency but apply all frequencies simultaneously. A conductive and nonconductive surfaces were investigated by EIS, using sinchronyzed SECM and FFTS and data fit to results of SECM aproach curves. The study of active and deactivated yeast cells using vitamin K group compounds as a redox mediator pair, enables to register oxygen consumption by alive cells, because vitamin K can diffuse into the cell and actively participate in electron transfer inside the cell. After evaluation of data obtained from the yeast cell investigation, the SECM different few modes were selected for the investigation of human heart left ventricle myocardium-derived mesenchymal stem cells (hmMSC) by SECM approach curves using vitamin K compounds as a redox mediator pair. Confirmly, the SECM approach curves method is suitable for recognizing different redox activity of healthy and pathological hmMSC. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Doctoral thesis |
| Language |
English |
| Publication date |
2020 |
