| Title |
Targeted RNA knockdown by a type III CRISPR-Cas complex in zebrafish / |
| Authors |
Fricke, Thomas ; Smalakytė, Dalia ; Lapinski, Maciej ; Pateria, Abhishek ; Weige, Charles ; Pastor, Michal ; Kolano, Agnieszka ; Winata, Ceclia ; Šikšnys, Virginijus ; Tamulaitis, Gintautas ; Bochtler, Matthias |
| DOI |
10.1089/crispr.2020.0032 |
| Full Text |
|
| Is Part of |
The CRISPR journal.. New Rochelle, NY : Mary Ann Liebert, Inc.. 2020, vol. 3, iss. 4, p. 299-313.. ISSN 2573-1599. eISSN 2573-1602 |
| Keywords [eng] |
CRISPR-Cas ; targeted RNA knockdown ; zebrafish |
| Abstract [eng] |
RNA interference is a powerful experimental tool for RNA knockdown, but not all organisms are amenable. Here, we provide a proof of principle demonstration that a type III Csm effector complex can be used for programmable mRNA transcript degradation in eukaryotes. In zebrafish, Streptococcus thermophilus Csm complex (StCsm) proved effective for knockdown of maternally expressed EGFP in germ cells of Tg(ddx4:ddx4-EGFP) fish. It also led to significant, albeit less drastic, fluorescence reduction at one day postfertilization in Tg(myl7:GFP) and Tg(fli1:EGFP) fish that express EGFP zygotically. StCsm targeted against the endogenous tdgf1 elicited the characteristic one-eyed phenotype with greater than 50% penetrance, and hence with similar efficiency to morpholino-mediated knockdown. We conclude that Csm-mediated knockdown is very efficient for maternal transcripts and can also be used for mixed maternal/early zygotic and early zygotic transcripts, in some cases reaching comparable efficiency to morpholino-based knockdown without significant off-target effects. |
| Published |
New Rochelle, NY : Mary Ann Liebert, Inc |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
