| Abstract [eng] |
The diversity of living conditions of microorganisms predict that the microbiota is a huge source of new biocatalysts. Metagenomic libraries are used for the screening and selection of metagenomics enzymes. In this study, selection methods for the screening of esterases, amidohydrolases and glycosidases from metagenomic libraries were developed. To develop the selection methods for hydrolases, synthetic nucleosides were chosen as the sole source of uridine, supporting the growth only of those recombinant clones, which encode hydrolases that complement the uridine auxotrophy of the E. coli DH10B ΔpyrFEC strain by hydrolysis of uridine analogues. The analysis of selected hydrolases showed, that the developed methods are suitable for the selection of metagenomic enzymes with unknown function and selected biocatalysts are very diverse in their sequences and catalytic properties. |
