| Abstract [eng] |
The artificial phospholipid membranes on solid surfaces have been extensively studied over the past few decades. Mostly gold surfaces have been investigated, however gold substrates are associated with significant cost for specimen as well as they are mostly disposable after a single use. Therefore, in this work, for the first time, mechanically polished metallurgical titanium and aluminum surfaces were investigated for artificial phospholipid membrane formation. The procedure of silane-based self-assembled monolayer formation, which is needed for phospholipid membrane formation, was established. The formation of the artificial phospholipid bilayers was accomplished via a simple vesicle-fusion method. Also, the self-assembled monolayers on Ti and Al surfaces were found to be reusable for multiple times for artificial phospholipid membrane formation. The biological relevance of artificial phospholipid membranes formed on Ti and Al was displayed using membrane damaging proteins/peptides. The results in this work show that artificial membranes on titanium and aluminum surfaces have possible applications towards cost-efficient membrane-based biosensor devices. |
