| Abstract [eng] |
Tissue engineering is an interdisciplinary field aimed at constructing artificial tissues and organs that could be used for transplantation. The aim of this work was to investigate strategies and techniques for bioactive scaffold fabrication for both hard and soft tissue types. For hard tissue, a novel hybrid organic-inorganic material containing Al has been developed and structured in 3D using direct laser writing. The material was then compared to other similar materials containing Ti, V and Zr in terms of its biocompatibility in vitro. Results show that the newly developed polymer is suitable for tissue engineering applications and improves collagen secretion in rat myogenic stem cells. Soft tissue engineering strategies were based on polydimethylsiloxane (PDMS). We demonstrated that its mechanical properties can be tuned by incorporating silk. In addition to that, a selection of PDMS block copolymers were screened in terms of their biocompatibility, some of which are promising for further biological applications. Surface biodecoration using collagen and RGD tripeptides significantly increased the number of attached rabbit myogenic stem cells and their metabolic activity. |
