| Abstract [eng] |
Combination therapy is the cornerstone of cancer treatment. The simultaneous application of cytotoxic drugs potentiates their efficacy compared with monotherapy because it targets the key pathways in a synergistic or an additive manner. Such therapy is likely to diminish drug resistance, while simultaneously providing cytotoxic benefits, such as inhibition of tumor growth, decrease of cancer stem cell population, reduction of metastatic potential, and induction of apoptosis. In the present study, we investigated the effects of two drugs – dichloroacetate and salinomycin and the combination of both agents on the colorectal as well as lung cancer cell lines in vitro in 2D and 3D cell culture models. We also have tested therapeutic potential of this combination therapy in vivo in an allograft C57BL/6 mice model. In vitro studies were dedicated to investigate potential mechanism of salinomycin+dichloroacetate action and with in vivo studies we analyzed effects of this therapy on tumor growth, metastatic site formation, and the expression of tumor progression markers. |
