| Title |
Photocage-selective capture and light-controlled release of target proteins / |
| Authors |
Rakauskaitė, Rasa ; Urbanavičiūtė, Giedrė ; Simanavičius, Martynas ; Lasickienė, Rita ; Vaitiekaitė, Aušra ; Petraitytė, Gražina ; Masevičius, Viktoras ; Žvirblienė, Aurelija ; Klimašauskas, Saulius |
| DOI |
10.1016/j.isci.2020.101833 |
| Full Text |
|
| Is Part of |
iScience.. Cambridge, MA : Cell Press. 2020, vol. 23, iss. 12, art. no. 101833, p. 1-28.. ISSN 2589-0042 |
| Keywords [eng] |
biochemistry ; biochemistry methods ; biotechnology ; proteins |
| Abstract [eng] |
Photochemical transformations enable exquisite spatiotemporal control over biochemical processes; however, methods for reliable manipulations of biomolecules tagged with biocompatible photo-sensitive reporters are lacking. Here we created a high-affinity binder specific to a photolytically removable caging group. We utilized chemical modification or genetically encoded incorporation of noncanonical amino acids to produce proteins with photocaged cysteine or selenocysteine residues, which were used for raising a high-affinity monoclonal antibody against a small photoremovable tag, 4,5-dimethoxy-2-nitrobenzyl (DMNB) group. Employing the produced photocage-selective binder, we demonstrate selective detection and immunoprecipitation of a variety of DMNB-caged target proteins in complex biological mixtures. This combined orthogonal strategy permits photocage-selective capture and light-controlled traceless release of target proteins for a myriad of applications in nanoscale assays. |
| Published |
Cambridge, MA : Cell Press |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
