MIRRAGGE – minimum information required for reproducible AGGregation experiments /

Pedro M Martins; Susanna Navarro; Alexandra Silva; Maria F Pinto; Francisco Sarkany; Pedro Jose Barbosa Pereira; Francisca Pinheiro; Zuzana Bednarikova; Michail Burdukiewicz; Oxana V Galzitskaya; Zuzana Gazova; Claudio M Gomez; Annalisa Pastore; Louise C Serpell; Rostislav Skrabana; Vytautas Smirnovas; Mantas Žiaunys; Daniel E Otzen; Salvador Ventura; Sandra Macedo-Ribeiro; Francisco Figueiredo

doi:10.3389/fnmol.2020.582488

Title	MIRRAGGE – minimum information required for reproducible AGGregation experiments /
Authors	Martins, Pedro M ; Navarro, Susanna ; Silva, Alexandra ; Pinto, Maria F ; Sarkany, Francisco ; Barbosa Pereira, Pedro Jose ; Pinheiro, Francisca ; Bednarikova, Zuzana ; Burdukiewicz, Michail ; Galzitskaya, Oxana V ; Gazova, Zuzana ; Gomez, Claudio M ; Pastore, Annalisa ; Serpell, Louise C ; Skrabana, Rostislav ; Smirnovas, Vytautas ; Žiaunys, Mantas ; Otzen, Daniel E ; Ventura, Salvador ; Macedo-Ribeiro, Sandra ; Figueiredo, Francisco
DOI	10.3389/fnmol.2020.582488
Full Text
Is Part of	Frontiers in molecular neuroscience.. Lausanne : Frontiers media SA. 2020, vol. 13, art. no 582488, p. [1-18].. ISSN 1662-5099
Keywords [eng]	amyloid ; reproducible data ; protein ; peptide ; phase separation
Abstract [eng]	Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.
Published	Lausanne : Frontiers media SA
Type	Journal article
Language	English
Publication date	2020
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„MIRRAGGE – minimum information required for reproducible AGGregation experiments /“