| Title |
Formation and electrochemical evaluation of polyaniline and polypyrrole nanocomposites based on glucose oxidase and gold nanostructures / |
| Authors |
German, Natalija ; Ramanavičienė, Almira ; Ramanavičius, Arūnas |
| DOI |
10.3390/polym12123026 |
| Full Text |
|
| Is Part of |
Polymers.. Basel : MDPI. 2020, vol. 12, iss. 12, art. no. 3026, p. 1-20.. eISSN 2073-4360 |
| Keywords [eng] |
biofuel cell ; cyclic voltammetry ; glucose biosensor ; glucose oxidase ; gold nanoparticles ; conducting polymers ; polyaniline ; polymer nanocomposite ; polypyrrole |
| Abstract [eng] |
Nanocomposites based on two conducting polymers, polyaniline (PANI) and polypyrrole (Ppy), with embedded glucose oxidase (GOx) and 6 nm size gold nanoparticles (AuNPs(6nm)) or gold-nanoclusters formed from chloroaurate ions (AuCl4−), were synthesized by enzyme-assisted polymerization. Charge (electron) transfer in systems based on PANI/AuNPs(6nm)-GOx, PANI/AuNPs(AuCl4−)-GOx, Ppy/AuNPs(6nm)-GOx and Ppy/AuNPs(AuCl4−)-GOx nanocomposites was investigated. Cyclic voltammetry (CV)-based investigations showed that the reported polymer nanocomposites are able to facilitate electron transfer from enzyme to the graphite rod (GR) electrode. Significantly higher anodic current and well-defined red-ox peaks were observed at a scan rate of 0.10 V s−1. Logarithmic function of anodic current (log Ipa), which was determined by CV-based experiments performed with glucose, was proportional to the logarithmic function of a scan rate (log v) in the range of 0.699–2.48 mV s−1, and it indicates that diffusion-controlled electrochemical processes were limiting the kinetics of the analytical signal. The most efficient nanocomposite structure for the design of the reported glucose biosensor was based on two-day formed Ppy/AuNPs(AuCl4−)-GOx nanocomposites. GR/Ppy/AuNPs(AuCl4−)-GOx was characterized by the linear dependence of the analytical signal on glucose concentration in the range from 0.1 to 0.70 mmol L−1, the sensitivity of 4.31 mA mM cm−2, the limit of detection of 0.10 mmol L−1 and the half-life period of 19 days. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
