| Title |
Multidrug-resistant Acinetobacter baumannii genetic characterization and spread in Lithuania in 2014, 2016, and 2018 / |
| Authors |
Kirtiklienė, Tatjana ; Mierauskaitė, Aistė ; Razmienė, Ilona ; Kuisienė, Nomeda |
| DOI |
10.3390/life11020151 |
| Full Text |
|
| Is Part of |
Life.. Basel : MDPI. 2021, vol. 11, iss. 2, art. no. 151, p. [1-12].. ISSN 2075-1729 |
| Keywords [eng] |
multidrug resistance ; nosocomial infections ; Acinetobacter baumannii ; MLVA-8 ; resistance genes |
| Abstract [eng] |
Bacterial resistance to antimicrobial agents plays an important role in the treatment of bacterial infections in healthcare institutions. The spread of multidrug-resistant bacteria can occur during inter- and intra-hospital transmissions among patients and hospital personnel. For this reason, more studies must be conducted to understand how resistance occurs in bacteria and how it moves between hospitals by comparing data from different years and looking out for any patterns that might emerge. Multidrug-resistant (MDR) Acinetobacter spp. was studied at 14 healthcare institutions in Lithuania during 2014, 2016, and 2018 using samples from human bloodstream infections. In total, 194 isolates were collected and identified using MALDI-TOF and VITEK2 analyzers as Acinetobacter baumannii group bacteria. After that, the isolates were analyzed for the presence of different resistance genes (20 genes were analyzed) and characterized by using the Rep-PCR and MLVA (multiple-locus variable-number tandem repeat analysis) genotyping methods. The results of the study showed the relatedness of the different Acinetobacter spp. isolates and a possible circulation of resistance genes or profiles during the different years of the study. This study provides essential information, such as variability and diversity of resistance genes, genetic profiling, and clustering of isolates, to better understand the antimicrobial resistance patterns of Acinetobacter spp. These results can be used to strengthen the control of multidrug-resistant infections in healthcare institutions and to prevent potential outbreaks of this pathogen in the future. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
