| Abstract [eng] |
In modern medicine the benefit of improvement of the collected experimental data specificity is self-evident. This leads to a better understanding of the complex mechanisms of diseases, increased sensitivity of diagnosis, and the overall higher quality treatment of the patients. Thus, new advanced research methods or novel applications for the conventional ones are created. One of these methods are vibrational spectroscopy methods. They are especially sensitive to the chemical changes and thus are increasingly applied for the chemical analysis of biological samples. However, because of several reasons the use of vibrational spectroscopy methods in clinical analysis is yet to become a common practice. One of the reasons is difficult interpretation of the spectra of complex samples. Another problem is determination of the optimal strategy of sample preparation for efficient analysis. Also, often the concentration of molecules in the samples under study is too low to be analysed via conventional vibrational spectroscopy methods. Then, non-conventional spectroscopic techniques like surface enhanced Raman scattering has to be used. Therefore, the main goal of the thesis is the development of novel vibrational spectroscopy application methods for the analysis of biological samples – tissues of the urinary tract cancers, bacteria cells, and biological fluids. The prime focus is to adapt the vibrational spectroscopy methods for analysis of biological and medical samples. |
