| Abstract [eng] |
Polypyrrole (Ppy) as suitable material for biosensor design and modification of living cells was investigated. Polypyrrole films were electrochemically synthesized and repulsion possibility of the Ppy film by potential cycling was considered. Comparison of DNA molecularly imprinted polypyrrole (MIPpy) formed by potential pulse sequence and potential cycling showed that MIPpy was formed by both methods, however potential pulse sequence was more suitable. Analysis of the caffeine MIPpy proved that such imprints are more sensitive to caffeine than to its homologue theophylline. Two types of Ppy composites were obtained: embedded with gold nanoparticles or enzyme glucose oxidase. After AuNP/Ppy composite synthesis no separate core shell nanoparticles were observed, but the composites of 100-200 nm were obtained. Ppy/enzyme composites were evaluated using Isotope ratio mass spectrometry and the ratio of initial materials was calculated. Biocompatibility experiments showed that Ppy is not toxic to mouse bone marrow-derived stem cells and do not impair cell attachment and proliferation. However, modification of the yeast cell itself with Ppy decreased viability by 26 – 40 %. The atomic force microscopy was used for the visualization of the yeast cell wall surface at high resolution. Raman spectroscopy analysis of yeast cells demonstrated that the substrate not only affects the intensity of the light hitting the substrate, but also the distribution of light intensity in the sample. |
