| Abstract [eng] |
The aim of the dissertation – to determine the histological response to hormonal treatment in boys with cryptorchidism and to summarize relevant gene expression changes. The research was performed on the histological and molecular data obtained during surgery from testicular biopsies of cryptorchid boys. Histological analysis was performed at the National Center of Pathology. Molecular studies were performed in Switzerland under the guidance of Prof. F. Hadziselimovič. Articles were published together with specialists united by the Cryptorchidism Research Institute (Liestal, Switzerland). It was determined that adjuvant hormonal treatment with gonadotropin-releasing hormone analogue (GnRHa) induces gonocyte transition to Ad spermatogonia – the stem cells for spermatogenesis. Differential gene expression significantly differs between high (HIR) and low (LIR) infertility risk groups that were determined by histological features. Analyzed genes are involved in hormonal control of hypothalamic-pituitary-gonadal axis and fertility, affect loci involved in canonical and alternative testosterone synthesis pathways. Moreover, non-coding RNAs that may play an important role in the regulation of spermatogenesis were analyzed. Investigated genes were significantly downregulated in HIR group and positively responded to GnRHa treatment. Based on the results adjuvant hormonal treatment with GnRHa is recommended for cryptorchid boys with a histologically confirmed HIR. Moreover, hypotheses for further prospective molecular and immunohistochemical studies of testicular biopsies are presented. |
