Title Molecular characterization and seroprevalence of hepatitis E virus in inflammatory Bowel disease patients and solid organ transplant recipients /
Authors Grigas, Juozas ; Montoya, Maria ; Šimkutė, Evelina ; Buitkus, Marius ; Zagrabskaitė, Rūta ; Pautienius, Arnoldas ; Razukevicius, Dainius ; Jonaitis, Laimas Virginijus ; Kiudelis, Gediminas ; Skiecevičienė, Jurgita ; Vaičiūnienė, Rūta ; Stankuvienė, Asta ; Bumblytė, Inga Arūnė ; Kupčinskas, Juozas ; Stankevičius, Arūnas
DOI 10.3390/v13040670
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Is Part of Viruses.. Basel : MDPI. 2021, vol. 13, no. 4, art. no. 670, p. [1-17].. eISSN 1999-4915
Keywords [eng] Crohn’s disease ; MARC-145 ; biological therapy ; genotype 3 ; hepatitis E ; immunosuppression ; inflammatory bowel disease ; solid organ transplant ; ulcerative colitis ; zoonosis
Abstract [eng] Seroprevalence rates and molecular characterization of hepatitis E virus (HEV) prevalent in the Lithuanian human population has not yet been evaluated. Immunosuppressed individuals have been recognized as a risk group for chronic hepatitis due to HEV genotype 3 (HEV-3) infections. The objectives of the present study were to determine prevalence rates of anti-HEV antibodies among inflammatory bowel disease (IBD) patients and solid organ transplant (SOT) recipients, to isolate and characterize HEV strain present in the Lithuanian human population, and to investigate its capacity to infect non-human primate (MARC-145 and Vero), swine (PK-15) and murine (Neuro-2a) cells in vitro. In the present study, the significant difference of anti-HEV IgG prevalence between healthy (3.0% (95% CI 0-6.3)) and immunosuppressed individuals (12.0% [95% CI 8.1-15.9]) was described. Moreover, our findings showed that anti-HEV IgG seropositivity can be significantly predicted by increasing age (OR = 1.032, p < 0.01), diagnosis of IBD (OR = 4.541, p < 0.01) and reception of SOT (OR = 4.042, <0.05). Locally isolated HEV strain clustered within genotype 3i subtype of genotype 3 and was capable of infecting MARC-145 cells. This study demonstrates higher HEV seroprevalence in the risk group compared to healthy control individuals without confidence interval overlap. The high level of genetic homology between human and animal strains in Lithuania and the capacity of locally isolated strains to infect cells of non-human origin suggests its potential for zoonotic transmission.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2021
CC license CC license description