| Abstract [eng] |
The ability of antibodies to specifically recognize various biomolecules is widely applied in various fields, including diagnostic and analytical methods, whereas antibodies immobilized on magnetic particles are widely used in enzyme-linked immunosorbent assays. Polyclonal antibodies used in the analysis exhibit batch-to-batch variability, and the use of monoclonal antibodies reduces the sensitivity of analytical methods. Recombinant antibody technologies can address these issues. The aim of this study was to develop analytical method for determining the amount of target molecules on magnetic beads by applying recombinant polyclonal antibodies. During the analysis, different libraries of recombinant polyclonal antibodies were tested. For each of target analyte one library was selected. The following optimization of the analytical method was conducted by applying full factorial analysis design. Based on obtained results the conditions of the assays were established. The optimized method was qualified according to the requirements. One of the two analysis methods was successfully qualified. |
