| Keywords [eng] |
Construction of biosensor, cyclic voltammetry, chronoamperometry, Prussian blue, Glucose, Glucose oxidase, ascorbic acid, L-cysteine, uric acid and paracetamol. |
| Abstract [eng] |
Enzyme based electrochemical biosensor are very important for the diagnosis of body fluids. The need of implantable biosensors for the detection of glucose in the body is becoming crucial day by day. The major problem in the detection of blood glucose is caused by the interfering substances present in the blood. Another problem is related with the choice of electrode used in previous researches. Despite of increasing the response current, the electrodes have poor electrochemical conductivity, instable operational ability and the cost is much high. Therefore, a project was designed to continue the research for the investigation of the effect of interfering substances on the sensitivity of enzyme-based biosensor for the detection of glucose. To make enzyme-based glucose biosensor PB was used as a transducer. The electrochemical deposition was done by CV on FTO. It was followed by the dispersion of GOX on the glass/FTO/PB. After the dispersion of enzyme, it was cross linked with 25% glutaraldehyde solution and glass/FTO/PB/GOX biosensor was constructed. In this research project four substances along with glucose were examined to see their influence on the sensitivity of the glass/FTO/PB/GOX biosensors. These substances were ascorbic acid, L-cysteine, uric acid and paracetamol. Among above-mentioned substances ascorbic acid and L-cysteine were found reactive towards the glass/FTO/PB/GOX biosensor, while uric acid and paracetamol showed no reactivity. In future, using the information from this project, further investigation can be done to modify PB based biosensor for the identification of glucose. So, it can be use in practical implantation as a diagnostic tool in diabetes. |
