| Abstract [eng] |
Application of Polydopamine in Biological Sensors The suitability of polydopamine (PDA) for design of electrochemical immune sensor for the detection of antibodies against bovine serum albumin (BSA) was investigated. The hydrophilicity of the sample increases due to PDA layer deposition on the ITO coated glass surface. In addition, the samples surface with PDA layer synthesized from 0,5 mg/mL dopamine solution was more hydrophilic than samples surface with PDA layer deposited from 0,1 mg/mL dopamine solution. Cyclic voltammetry measurements showed that the diffusion coefficient of potassium hexacyanoferrate decreases after deposition of PDA layer on ITO coated glass. Differential pulse voltammetry measurements showed that current intensity at the peak decreases after immobilization of biomolecules on the samples surface. Changes in current intensity at the peak depends on the concentration of BSA protein solution. The linear dependence was observed in range from 5 to 100 μg/mL. The optimal BSA protein solution concentration for immobilization is 100 μg/mL. Comparing the BSA immobilization methods, it was found that immobilization of BSA using crosslinking with glutaraldehyde vapor is the most suitable method for fabrication of immunosensor for the specific detection of Anti-BSA antibodies. It was observed that changes in current intensity at peak linearly depends on the concentration of Anti-BSA solution concentration in range from 2,5 to 15 μg/mL using glutaraldehyde vapor for BSA immobilization. After modification of ZnO nanorods with PDA layer and immobilization of BSA protein using glutaraldehyde changes of current intensity at the peak were linearly dependent on the concentration of Anti-BSA antibodies concentration in range from 2,5 to 10 μg/mL. |
