| Abstract [eng] |
Master's thesis of D. Mačiežaitė "Designing and Qualitative Evaluation of a Semi-solid Pharmaceutical Form With Licorice (Glycyrrhiza glabra) Root Extract". Scientific supervisor assistant Dr. R. Kalėdaitė; Lecturer R. Matulaitienė was research consultant; Vilnius University, Faculty of Medicine, Institute of Biomedical Sciences (Pharmacy and Pharmacology Center), Vilnius 2024. Aim of the study: to not only produce stable semi-solid pharmaceutical forms with licorice root extract, but also to study and evaluate their qualitative properties and stability. Objectives: 1. Select ingredients suitable for production and design stable semi-solid pharmaceutical forms. 2. Evaluate the qualitative characteristics of the produced semi-solid pharmaceutical forms by determining the type, pH and viscosity. 3. Evaluate the stability of semi-solid pharmaceutical forms 1, 2, 3, and 4 weeks after production. 4. Evaluate the organoleptic properties of the produced semi-solid pharmaceutical forms by interviewing the respondents who tested them. Methods: the production of licorice root liquid extract from raw material of pharmaceutical grade plant; the designing of a semi-solid pharmaceutical form using a mixer; the evaluation of the type of manufactured semi-solid pharmaceutical forms, determination of pH and viscosity; the study of stability of designed pharmaceutical forms under room temperature storage conditions 1, 2, 3, and 4 weeks after production. Results: 12 different semi-solid pharmaceutical forms were formulated with licorice root extract after choosing the right materials. They were marked with identification numbers from E 2 to E 13 and their qualitative characteristics and stability were investigated. Research findings: 1. Selected materials suitable for modeling semi-solid pharmaceutical forms. 11 stable semi-solid pharmaceutical forms with licorice root ethanolic-water extract (30:70) were produced. 2. The o/w type was determined for all designed semi-solid pharmaceutical forms. After 4 weeks, the semi-solid pharmaceutical forms E 2, E 3, E 4, E 5, E 6, E 7, E 11 and E 12 became more acidic compared to the results of the first week (p < 0.05), E 8 pH and E 9 pH remained the same (p > 0.05), while E 10 became more alkaline (p < 0.05). The viscosity of the designed pharmaceutical forms was determined at different temperatures: 2 °C, 15 °C, 22 °C and 40 °C. The amount of water in the composition did not affect the viscosity (r = 0). Viscosity at temperatures below 15 °C was influenced by licorice root (r = -0.32) and fat content (r = -0.20) in the pharmaceutical form. Above 15 °C, the quantity of emulsifiers had an effect on viscosity (r = 0.77). 3. The semi-solid pharmaceutical forms remained stable for 4 weeks after production and remained stable when centrifuged at 3000, 6000, and 9000 rpm, except for sample E 6, where the oil and aqueous phases separated. 4. The respondents with dry skin type rated the colors of products A and B with a higher score compared to those, whose skin tends to dry (p < 0.05). 5 minutes after using the B semi-solid pharmaceutical form, respondents with dry skin felt a greater sense of hydration compared to those with normal skin or those, whose skin tends to dry (p < 0.05). Also, 5 minutes after using the B semi-solid pharmaceutical form, respondents from age groups of 66 to 78 years old and of 40 to 65 years old felt a greater sense of stickiness compared to the age group from 25 to 39 years old (p < 0.05). All respondents rated the color of A and B semi-solid pharmaceutical forms at least 4 points (p < 0.05). The respondents evaluate the smell and the consistency of A and B semi-solid pharmaceutical forms equally (p > 0.05). |
