| Abstract [eng] |
Glioma, a type of brain cancer, poses significant challenges due to its aggressive nature and limited treatment options. Its infiltrative growth pattern and resistance to therapy make it particularly difficult to manage, emphasizing the urgent need for innovative research approaches. Microfluidic chips offer a promising avenue for studying glioma behavior in a controlled environment. With their versatile design and fabrication capabilities, microfluidic chips allow a precise manipulation of fluid behavior at the microscale. Therefore, understanding the importance of cell adhesion within microfluidic chip is crucial, as it plays a pivotal role in the invasive behavior and treatment response. The fabrication of the microfluidic chip involved a multi-step process, including 3-D printing of master molds, PDMS layer casting, preparation of COC substrates, and injection casting of a photo-curable resin – OSTE. Polycarbonate membranes were placed onto the COC, and the chip was assembled with precise alignment of COC substrates with patterned relief structures. For adhesion modification, microfluidic channels were coated with various solutions, including gelatin 38, gelatin 175, gelatin 300, a mix of gelatin 175/collagen, and collagen. In the experiment C6 glioma rat cells were used. Prepared solutions were injected into the channels of the microfluidic chips and glioma cells were seeded into the channels afterwards. Over the course of one week, the cells were cultured within the microfluidic environment and imaged using microscopy before and after exposure to fluid flow from the pump. Adhesion behavior of the glioma cells was assessed, with a particular focus on quantifying adherent and non-adherent cells between trials and different adhesion modification methods. Investigation into microfluidic pump impact on C6 glioma cell adhesion showed variations in cell counts between treatment groups before and after exposure to the microfluidic environment. However, comparison of cell counts between different adhesion modifications revealed that gelatin coatings resulted in decreased adherent cell counts, while collagen and mixed modification methods exhibited higher counts. In conclusion, these results suggest that collagen may be utilized to enhance C6 glioma cell adhesion. |
