| Title |
dGAE(297-391) tau fragment promotes formation of chronic traumatic encephalopathy-like tau filaments / |
| Authors |
Kitoka, Kristine ; Lends, Alons ; Kucinskas, Gytis ; Bula, Anna Lina ; Krasauskas, Lukas ; Smirnovas, Vytautas ; Zilkova, Monika ; Kovacech, Branislav ; Skrabana, Rostislav ; Hritz, Jozef ; Jaudzems, Kristaps |
| DOI |
10.1002/anie.202407821 |
| Full Text |
|
| Is Part of |
Angewandte chemie-international edition.. Weinheim : Wiley-VCH GmbH. 2024, first published online, art. no. e202407821, p. [1-8].. ISSN 1433-7851. eISSN 1521-3773 |
| Keywords [eng] |
NMR spectroscopy ; dGAE fragment ; filamentous aggregates ; tau protein ; tauopathies |
| Abstract [eng] |
The microtubule-associated protein tau forms disease-specific filamentous aggregates in several different neurodegenerative diseases. In order to understand how tau undergoes misfolding into a specific filament type and to control this process for drug development purposes, it is crucial to study in vitro tau aggregation methods and investigate the structures of the obtained filaments at the atomic level. Here, we used the tau fragment dGAE, which aggregates spontaneously, to seed the formation of full-length tau filaments. The structures of dGAE and full-length tau filaments were investigated by magic-angle spinning (MAS) solid-state NMR, showing that dGAE allows propagation of a chronic traumatic encephalopathy (CTE)-like fold to the full-length tau. The obtained filaments efficiently seeded tau aggregation in HEK293T cells. This work demonstrates that in vitro preparation of disease-specific types of full-length tau filaments is feasible. |
| Published |
Weinheim : Wiley-VCH GmbH |
| Type |
Journal article |
| Language |
English |
| Publication date |
2024 |
| CC license |
|
