| Abstract [eng] |
The objectives of this efforts was to develop spectroscopic ellipsometry method for investigation of optical properties: (i) of multi-layered nanostructures used for Bovine leukaemia virus glycoprotein gp51 recognition, (ii) nanostructures consisting of monomeric (mGCSF) and dimeric (dGCSF) granulocyte colony stimulating factor and its receptor (GCSF-R) and (iii) Al2O3/ZnO nanolaminates designed by atomic layer deposition method. In particular, focusing on the investigation of: • biological recognition layers of immunosensors by means of comparison of analytical sensitivities of ellipsometric parameters Δ and Ψ in order to estimate differences in orientation of immobilized intact- and fragmented-antibodies against bovine leukemia virus antigen gp51; • the kinetics of biosensing layer formation, which was based on the immobilization of fragmented and intact antibodies, kinetics of antigen interaction with the immobilized antibodies and interactions of monomeric (mGCSF) and dimeric (dGCSF) granulocyte colony stimulating factor and its receptor (GCSF-R) immobilized on a thin gold layer; • kinetic coefficients of monomolecular biological layer formation process using model which takes into consideration partial reversibility (residence time) effect; • optical constants of Al2O3 and ZnO monolayer as well as Al2O3/ZnO nanolaminates prepared by atomic layer deposition method in order to investigate possibilities to apply these nanolaminates for optical biosensors. |
