Title Overcoming antimicrobial resistance in bacteria using bioactive magnetic nanoparticles and pulsed electromagnetic fields /
Authors Novickij, Vitalij ; Stanevičienė, Ramunė ; Vepštaitė-Monstavičė, Iglė ; Gruškienė, Rūta ; Krivorotova, Tatjana ; Sereikaitė, Jolanta ; Novickij, Jurij ; Servienė, Elena
DOI 10.3389/fmicb.2017.02678
Full Text Download
Is Part of Frontiers in microbiology.. Lausanne : Frontiers Research Foundation. 2018, vol. 8, 2678, p. 1-8.. ISSN 1664-302X
Keywords [eng] Antimicrobial resistance ; Bacteria inactivation ; B. subtilis ; E. coli ; Iron oxide nanoparticles ; Nisin
Abstract [eng] Nisin is a known bacteriocin, which exhibits a wide spectrum of antimicrobial activity, while commonly being inefficient against Gram-negative bacteria. In this work, we present a proof of concept of novel antimicrobial methodology using targeted magnetic nisin-loaded nano-carriers [iron oxide nanoparticles (NPs) (11–13 nm) capped with citric, ascorbic, and gallic acids], which are activated by high pulsed electric and electromagnetic fields allowing to overcome the nisin-resistance of bacteria. As a cell model the Gram-positive bacteria Bacillus subtilis and Gram-negative Escherichia coli were used. We have applied 10 and 30 kV cm-1 electric field pulses (100 μs × 8) separately and in combination with two pulsed magnetic field protocols: (1) high dB/dt 3.3 T × 50 and (2) 10 mT, 100 kHz, 2 min protocol to induce additional permeabilization and local magnetic hyperthermia. We have shown that the high dB/dt pulsed magnetic fields increase the antimicrobial efficiency of nisin NPs similar to electroporation or magnetic hyperthermia methods and a synergistic treatment is also possible. The results of our work are promising for the development of new methods for treatment of the drug-resistant foodborne pathogens to minimize the risks of invasive infections.
Published Lausanne : Frontiers Research Foundation
Type Journal article
Language English
Publication date 2018