| Abstract [eng] |
Identification and Application of Informative Genetic Markers in Studies of Genetic Diversity of Experimental and Wild Populations of Lemna minor The aim of the study was to measure the genetic diversity of wild population L. minor clones using different genetic markers and the genetic variability in experimental clones exposed to increased electromagnetic radiation by GPx, Cat and APx gene sequencing. Materials and methods - The wild type L. minor samples from Nemunas River near Kaunas (4 samples) and Neris River near Vilnius (8 samples) were collected. The genetic diversity of the wild type L. minor was studied using the genetic markers of the chloroplast genome including microsatellite sequences surrounded by non-coding regions and the antioxidant genes (GPx, Cat, APx). The growth parameters and DNA sequences of genetic markers were measured of L. minor grown in Petri dishes placed at different distances from the source of electromagnetic radiation. Results - Seven genetic markers (GPx6, GPx7, Cat4b, Cat4b, Cat7, APx1, APx2) were identified as suitable for wild type and experimental L. minor clones genetic diversity studies. Results indicate that the interpopulation diversity identified using microsatellite markers is higher (30% (L4), 29% (L14), 33% (L16)) compared to genetic markers encompassing fragments of antioxidant genes (5% (GPx6), 10% (GPx7), 15 % (Cat4-Cat4b), 10% (Cat7), 19% (APx1-APx2)). Growth of plants were suppressed after 14th week from the beginning of the exposure to low-frequency (50 Hz) electromagnetic radiation and new nucleotide varations in DNA sequences of antioxidant genes were inducted. |
