Obesity-associated deficits in inhibitory control are phenocopied to mice through gut microbiota changes in one-carbon and aromatic amino acids metabolic pathways /

Maria Arnoriaga-Rodríguez; Jordi Mayneris-Perxachs; Oren Contreras-Rodríguez; Aurelijus Burokas; Juan-Antonio Ortega-Sanchez; Gerard Blasco; Claudia Coll; Carles Biarnés; Anna Castells-Nobau; Josep Puig; Josep Garre-Olmo; Rafael Ramos; Salvador Pedraza; Ramon Brugada; Joan C Vilanova; Joaquín Serena; Jordi Barretina; Jordi Gich; Vicente Pérez-Brocal; Andrés Moya; Xavier Fernández-Real; Lluis Ramio-Torrentà; Reinald Pamplona; Joaquim Sol; Mariona Jové; Wifredo Ricart; Manuel Portero-Otin; Rafael Maldonado; Jose Manuel Fernández-Real

doi:10.1136/gutjnl-2020-323371

Title	Obesity-associated deficits in inhibitory control are phenocopied to mice through gut microbiota changes in one-carbon and aromatic amino acids metabolic pathways /
Authors	Arnoriaga-Rodríguez, Maria ; Mayneris-Perxachs, Jordi ; Contreras-Rodríguez, Oren ; Burokas, Aurelijus ; Ortega-Sanchez, Juan-Antonio ; Blasco, Gerard ; Coll, Claudia ; Biarnés, Carles ; Castells-Nobau, Anna ; Puig, Josep ; Garre-Olmo, Josep ; Ramos, Rafael ; Pedraza, Salvador ; Brugada, Ramon ; Vilanova, Joan C ; Serena, Joaquín ; Barretina, Jordi ; Gich, Jordi ; Pérez-Brocal, Vicente ; Moya, Andrés ; Fernández-Real, Xavier ; Ramio-Torrentà, Lluis ; Pamplona, Reinald ; Sol, Joaquim ; Jové, Mariona ; Ricart, Wifredo ; Portero-Otin, Manuel ; Maldonado, Rafael ; Fernández-Real, Jose Manuel
DOI	10.1136/gutjnl-2020-323371
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Is Part of	Gut.. London : BMJ Publishing Group. 2021, vol. 70, iss. 12, p. 2283-2296.. ISSN 0017-5749. eISSN 1468-3288
Keywords [eng]	intestinal microbiology ; microbiota ; obesity.
Abstract [eng]	Background: Inhibitory control (IC) is critical to keep long-term goals in everyday life. Bidirectional relationships between IC deficits and obesity are behind unhealthy eating and physical exercise habits. Methods: We studied gut microbiome composition and functionality, and plasma and faecal metabolomics in association with cognitive tests evaluating inhibitory control (Stroop test) and brain structure in a discovery (n=156), both cross-sectionally and longitudinally, and in an independent replication cohort (n=970). Faecal microbiota transplantation (FMT) in mice evaluated the impact on reversal learning and medial prefrontal cortex (mPFC) transcriptomics. Results: An interplay among IC, brain structure (in humans) and mPFC transcriptomics (in mice), plasma/faecal metabolomics and the gut metagenome was found. Obesity-dependent alterations in one-carbon metabolism, tryptophan and histidine pathways were associated with IC in the two independent cohorts. Bacterial functions linked to one-carbon metabolism (thyX,dut, exodeoxyribonuclease V), and the anterior cingulate cortex volume were associated with IC, cross-sectionally and longitudinally. FMT from individuals with obesity led to alterations in mice reversal learning. In an independent FMT experiment, human donor's bacterial functions related to IC deficits were associated with mPFC expression of one-carbon metabolism-related genes of recipient's mice. Conclusion: These results highlight the importance of targeting obesity-related impulsive behaviour through the induction of gut microbiota shifts.
Published	London : BMJ Publishing Group
Type	Journal article
Language	English
Publication date	2021
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„Obesity-associated deficits in inhibitory control are phenocopied to mice through gut microbiota changes in one-carbon and aromatic amino acids metabolic pathways /“